Summary
This course concerns modern bioanalytical techniques to investigate biomolecules both in vitro and in vivo, including recent methods to image, track and manipulate single molecules. We cover the basic principles of the respective methods and discuss examples from the current scientific literature.
Content
Techniques to monitor the function of single biomolecules and complexes
- Single molecule fluorescence spectroscopy (FRET, confocal and total internal reflection fluorescence microscopy)
- Force spectroscopy to monitor function of single proteins and cells
- Microscopy beyond the diffraction limit: Super-resolution microscopy
Surface sensors to elucidate and quantify molecular interactions
- Immobilizing biopolymers on surfaces
- Optical & electrical detection techniques
Development and application of microfluidic and nanofluidic sensor devices
- Miniaturization of analytical techniques: Lab on a chip
- Chemical and biochemical sensors
- Next-generation DNA sequencing approaches
Engineered biomolecules to manipulate cells or as drug delivery vehicles
- Nano-containers for drug delivery vectors
- DNA based self-assembly and nanofabrication of complex structures
- Professor: Angela Steinauer
- Teaching assistant: Georges Barnikol
- Teaching assistant: Linda Wedemann
