The goal of this course is to provide an overview on recent developments in the design and synthesis of fluorescent and bioluminescent probes for applications in basic research and medicine. Through the discussion of recently published advances, general design principles will be reviewed

Summary

This course will have three components: Molecular Simulation, Electronic Laboratory Notebooks, and Machine learning. The focus of the course is applications to microporous materials (metal organic frameworks, zeolites, covalent organic frameworks) and is aimed at non-computational chemists.

Towards Sustainable Chemistry: The development of efficient, atom and step economical processes is essential for the chemistry of the future. This training will empowered the student with all the tools of modern chemistry, which will be highly useful for his potential career as a process or medicinal chemist in industry.

The goal is to provide students with a complete overview of the principles and key applications of modern mass spectrometry and meet the current practical demand of EPFL researchers to improve structural analsis of molecules. Numerous instrumental aspects of mass spectrometry are described.

Content

The course program includes:

  • Mass spectrometry basics (ionization techniques, mass analyszers and detectors) and applications
  • Tandem mass spectrometry and hyphenation with separation (UPLC and GC) systems
  • Fragmentation mechanisms/techniques and structural analsis for identification of compounds
  • Fourier-Transform Mass spectrometry (Orbitrap and ICR mass analyzers)
  • Quantification (absolute and relative) methods with low resolution (triple quadrupole) and high-resolution (QTOF and Orbitrap) instruments
  • Analysis of biomolecules such as bottom-up and top-down MS for peptide/protein analysis, analysis of binding sites of metallated-ligands on peptides…
  • Open-source tools for Advanced analysis of mass spectra
  • Proteomics, lipidomics and metabolomics fields

 

 The course includes practical work in mass spectrometry that will be given mostly in the Mass Spectrometry Service Facility of ISIC (SSMI, SB, EPFL) and in the Proteomic Core Facility (PCF, SV, EPFL).